Study on application effect of fine needle aspiration cytology combined with multi-gene detection in diagnosis of thyroid nodules
10.3969/j.issn.1671-8348.2024.11.019
- VernacularTitle:细针穿刺细胞学联合多基因检测在甲状腺结节诊断中的应用效果研究
- Author:
Yanying NI
1
;
Ping SHEN
;
Zhongcai JIANG
Author Information
1. 航空总医院病理科,北京 100012
- Keywords:
thyroid nodules;
fine needle aspiration cytology;
BRAF V600E gene;
RAS gene
- From:
Chongqing Medicine
2024;53(11):1695-1699,1704
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression characteristics of BRAF,RAS and TERT genes in ultrasound-guided fine needle aspiration specimens and their application value in the preoperative diagnosis of thyroid nodules.Methods A retrospective analysis was performed on 537 cases of fine needle aspiration speci-mens of thyroid nodules in the Aviation General Hospital.The cell smear and liquid-based cell section prepara-tion from each specimen conducted the Papanicolaou's stain and the results were interpreted according to the Bethesda grading system.The gene mutation situation in punctured tissues was analyzed by real-time quantita-tive PCR.Results There were 65 cases (12.10％) of Bethesda class Ⅰ nodules,153 cases (28.49％) of class Ⅱ,84 cases (15.64％) of class Ⅲ,9 cases (1.68％) of class Ⅳ,73 cases (13.59％) of class Ⅴ and 153 cases (28.49％) of class Ⅵ.There were 220 cases (40.97％) of BRAF V600E mutation,21 cases (3.91％) of NRAS mutation,6 cases (1.12％) of KRAS mutation and 2 cases (0.37％) of HRAS mutation.Among them,there was BRAF V600E and KRAS co-mutation in 1 case.BRAF V600E gene mutation had the correlation with cytological diagnostic results (P<0.05),BRAF V600E mutation was more likely to occur in younger ones (P<0.05),and BRAF V600E mutation was more common in males than in females (P<0.05).KRAS mutation was more likely to occur in nodules in the left lobe of the thyroid gland (P<0.05).Conclusion BRAF V600E gene mutation is the most common among thyroid fine needle puncture specimens,and the NRAS mu-tation is in the majority for RAS gene mutations.
