Synergistic sensitization of apatinib mesylate and radiotherapy on hepatocarcinoma cells in vitro
10.13481/j.1671-587X.202404015
- VernacularTitle:甲磺酸阿帕替尼联合放疗对肝癌HepG2细胞的体外协同增敏作用
- Author:
Yongjing YANG
1
;
Tianyang KE
;
Shixin LIU
;
Xue WANG
;
Dequan XU
;
Tingting LIU
;
Ling ZHAO
Author Information
1. 吉林省肿瘤医院放疗一科,吉林长春 130012
- Keywords:
Hepatocellular carcinoma;
Apatinib mesylate;
Radiotherapy;
Cell proliferation;
Apoptosis;
Cell migration;
Vascular endothelial growth factor
- From:
Journal of Jilin University(Medicine Edition)
2024;50(4):1009-1015
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To discuss the synergistic inhibitory effect of apatinib mesylate(apatinib)combined with radiotherapy(RT)on the hepatocellular carcinoma(HepG2)cells in vitro,and to clarify its related antitumor mechanism.Methods:The HepG2 cells were cultured in vitro and treated with different concentrations of apatinib and/or varying doses of X-rays.MTT method was used to detect the survival rates of the cells in various groups;the inhibitory rates of cell proliferation and the 20％inhibitory concentration(IC20)of apatinib were calculated;the X-ray irradiation dose for subsequent experiments was detected.The HepG2 cells were divided into apatinib group,RT group,and apatinib+RT group(combined group).Flow cytometry was used to detect the apoptotic rates of the cells in various groups;wound healing assay was used to detect the migration rates of the cells in various groups;ELISA method was used to detect the levels of vascular endothelial growth factor(VEGF)in the cell culture supernatant in various groups.Results:The MTT results showed that the IC20 of apatinib was 1.32 μmol·L-1,and this concentration was used for subsequent experiments,and the X-ray irradiation dose for the follow-up experiments was 2 Gy.Compared with control group,the apoptotic rates of the cells in apatinib group and RT group had no significant differences(P＞0.05),while the apoptotic rate of the cells in combined group was increased(P＜0.05).Compared with control group,the migration rates of the cells in apatinib group,RT group,and combined group were decreased(P＜0.05);compared with apatinib group and RT group,the migration rate of the cells in combined group was decreased(P＜0.05).Compared with control group,the levels of VEGF in the cell culture supernatant in apatinib group and combined group were decreased(P＜0.05);compared with apatinib and RT group,the level of VEGF in the cell culture supernatant in combined group was decreased(P＜0.05).Conclusion:Apatinib combined with radiotherapy significantly inhibits the proliferation and migration of the HepG2 cells in vitro and induces the apoptosis;its effect may be related to the inhibition of VEGF secretion by cells.
