Immunomodulatory mechanism of umbilical cord mesenchymal stem cells modified by miR-125b-5p in systemic lupus erythematosus
10.19723/j.issn.1671-167X.2024.05.017
- VernacularTitle:miR-125b-5p修饰脐带间充质干细胞对系统性红斑狼疮的免疫调控机制
- Author:
Zhihui WU
1
;
Mingzhi HU
;
Qiaoying ZHAO
;
Fengfeng LV
;
Jingying ZHANG
;
Wei ZHANG
;
Yongfu WANG
;
Xiaolin SUN
;
Hui WANG
Author Information
1. 包头医学院第一附属医院中心实验室(内蒙古自治区自体免疫学重点实验室),内蒙古自治区包头 014010
- Keywords:
Mesenchymal stem cells;
Gene modification;
Systemic lupus erythematosus;
Immunomo-dulation;
Umbilical cord
- From:
Journal of Peking University(Health Sciences)
2024;56(5):860-867
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanism of immunomodulatory effects of umbilical cord mesenchymal stem cells(UC-MSCs)modified by miR-125b-5p on systemic lupus erythematosus(SLE).Methods:The expression level of miR-125b-5p was detected by real-time fluorescence quantitative PCR in UC-MSCs and peripheral blood mononuclear cells(PBMCs)from SLE patients and health checkers.Annexin V-FITC/PI apoptosis detection kit was used to detect the effect of miR-125b-5p on apoptosis of UC-MSCs.MRL/lpr mice in each group were injected with UC-MSCs via tail vein,and T-lymphocyte subsets in the spleen of the MRL/lpr mice were detected by flow cytometry after 5 weeks.The expression levels of interleukin(IL)-4 and IL-17A in serum of MRL/lpr mice were detected by ELISA.Hematoxylin-eosin staining was used to observe the pathological manifestations of the lungs and kidneys of the MRL/lpr mice.Results:miR-125b-5p was significantly down-regulated in PBMCs of SLE patients compared with healthy controls(P＜0.01).Compared with the UC-MSCs group,the expression of miR-125b-5p in UC-MSCs modified by miR-125b-5p group was increased(P＜0.01).The survival rate of UC-MSCs was significantly increased by miR-125b-5p(P＜0.01).Compared with the untreated group of MRL/lpr mice,the expression level of IL-4 in serum was increased(P＜0.05);the expression level of IL-17A was decreased(P＜0.05);the proportion of Th17 cells in the spleen of MRL/lpr mice was decreased(P＜0.05);the inflammatory cells infiltration and micro-thrombosis of lungs and kidneys of MRL/lpr mice were significantly reduced in the UC-MSCs modified by miR-125b-5p treatment group.Conclusion:UC-MSCs modified by miR-125b-5p have immunomodulatory effects on systemic lupus erythematosus.
