CLONING AND SEQUENCING OF COAT PROTEIN GENE FROM BARLEY YELLOW DWARF VIRUR GAV VIRUS
10.3321/j.issn:1000-8721.1999.04.012
- VernacularTitle:大麦黄矮病毒GAV株系外壳蛋白基因的克隆和序列分析
- Author:
Sheng-Jun CHANG
1
;
Xi-Feng WANG
;
Zhan-Hong MA
;
Li LI
;
Guang-He ZHOU
Author Information
1. 中国农业科学院植物保护研究所
- Keywords:
Barley yellow dwarf virus GAV;
Coat protein gene;
Sequence
- From:
Chinese Journal of Virology
1999;15(4):368-371
- CountryChina
- Language:Chinese
-
Abstract:
The Barley yellow dwarf virus (BYDV) GAV isolate was preserved at the Institute of Plant Protection of the Chinese Academy of Agricultural Sciences. The cDNA of BYDV GAV coat protein (CP) gene was amplified from the extracted RNA of BYDV GAV by using the polymerase chain reaction (PCR), and cloned into pGEM-7zf(+). Its complete nucleotide sequence has been determined by means of Sanger's dideoxy-mediated chain-termination method. The result showed that BYDV GAV CP gene has 600nt. It shares 97.5% and 96.5% identity with CP gene of BYDV MAV-PS1 in terms of nucleotide and amino acid sequences respectively.
