The root canal microbiota in apical periodontitis and pulpitis based on 16S rDNA sequencing
10.19405/j.cnki.issn1000-1492.2024.09.025
- VernacularTitle:基于16S rDNA测序的慢性牙髓炎及根尖周炎感染根管内菌群多样性研究
- Author:
Yuzhi LI
1
,
2
;
Xu SU
;
Xiaotao CHEN
;
Jie XU
;
Li ZHAO
Author Information
1. 石河子大学医学院口腔医学系,石河子 832000
2. 新疆维吾尔自治区人民医院口腔科,乌鲁木齐 830000
- Keywords:
pulpitis;
apical periodontitis;
microbiome;
oral flora;
16S rDNA;
sequencing
- From:
Acta Universitatis Medicinalis Anhui
2024;59(9):1669-1674,1687
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the relationship between microorganisms and endodontic disease by using 16S rDNA sequencing to compare the composition of the microbial community in the root canals of teeth with pulpitis and apical periodontitis.Methods Clinical samples were collected from teeth requiring root canal treatment.The to-tal DNA of the bacteria in the samples and the gene fragments of the V3-V4 highly variable region on the 16S rDNA fragments were amplified through PCR.After sequencing by NovaSeq,statistical and bioinformatic analysis,inclu-ding phylogenetic analysis,diversity analysis and analysis of group differences,were performed.Results In total,6 teeth with pulpitis and 7 teeth with apical periodontitis were collected,and a total of 8 510 OTUs were obtained after next-generation sequencing,and the analysis of bacterial diversity showed that the difference between pulpitis and apical periodontitis in terms of the composition of the bacterial flora was statistically significant(P＜0.05).In particular,the relative abundance of Proteobacteria,Acidobacteriota and Actinobacteriota phylum was significantly higher in the roots of teeth affected by pulpitis than apical periodontitis.The relative abundance of Bacteroidota phylum and Synergistota phylum was significantly higher in the root canals of teeth with apical periodontitis.Con-clusion There is a complex diversity of infecting microorganisms in the root canals of teeth affected by endodontic diseases.The microbial communities in the infected root canals of pulpitis and apical periodontitis show some differ-ences,and changes in the microbial composition of the root canals may be associated with the development of endo-dontic diseases.
