Mutations of the Pre-S Region in HBV-Associated Liver Cirrhosis and Hepatocellular Carcinoma.
- Author:
Sang Ook LEE
1
;
Youn Jae LEE
;
Yeun Sik JANG
;
Sang Hyuk LEE
;
Sang Young SEOL
;
Young Hong PARK
;
Jung Myung CHUNG
Author Information
1. Department of Internal Medicine, In Je University College of Medicine.
- Publication Type:Original Article
- Keywords:
Hepatitis/Viral/Hepatitis B;
Pre-S mutants;
Liver cirrhosis;
Neoplasm/Liver/Hepatocellular carcinoma
- MeSH:
Carcinoma, Hepatocellular*;
DNA;
Hepatitis B;
Humans;
Liver Cirrhosis*;
Liver Diseases;
Liver*;
Point Mutation;
Polymerase Chain Reaction
- From:The Korean Journal of Hepatology
2000;6(1):91-101
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND/AIMS: According to the recent research, mutations in the HBV pre-S region may have an impact on the progression of hepatitis B virus(HBV)-related liver disease. The aim of this study was to clarify the frequency and location of naturally occurring mutations in the pre-S region of HBV, and their possible effects on the clinical course of HBV-associated chronic liver diseases. METHODS: HBV DNA was extracted from the sera of 15 patients (8 with liver cirrhosis and 7 with hepatocellular carcinoma). The pre-S sequence was amplified via polymerase chain reaction, subcloning and sequenced. RESULTS: All patients had point mutations in the pre-S region. Nine of 10 mutation sites (90%) in the pre-S1 region, and 4 of 5 mutation sites (80%) in the pre-S2 region were identical in both liver cirrhosis and hepatocellular carcinoma. Deletions were detected in seven patients (4 with liver cirrhosis and 3 with hepatocellular carcinoma). Among the 4 patients with liver cirrhosis, three had deletion in 5'-end of the pre-S2 region and one spanning the 3'-end of the pre-S1 to 5'-end of the pre-S2 region. All 3 patients with hepatocellular carcinoma had deletions in 5'-end of the pre-S1 region, and two patients had simultaneous deletion spanning the 3'-end of the pre-S1 to the 5'-end of the pre-S2. CONCLUSION: The pre-S mutants were frequently detected in HBV-associated liver cirrhosis or hepatocellular carcinoma and the point mutations or deletions in the pre-S gene were clustered in specific regions.
