The expression and tyrosine phosphorylation of E-cadherin, beta-, gamma-catenin and EGFR after treatment of EGF and TGF-alpha in Cervical Cancer Cell Lines.
- Author:
Hye Sung MOON
;
Eun Ah CHOI
;
Hye Young PARK
- Publication Type:Original Article
- Keywords:
cervical cancer;
E-cadherin;
beta- and gamma-catenin;
EGFR
- MeSH:
Blotting, Western;
Cadherins*;
Cell Culture Techniques;
Cell Line*;
Epidermal Growth Factor*;
Epithelial Cells;
gamma Catenin*;
Immunoprecipitation;
Intercellular Signaling Peptides and Proteins;
Membranes;
Neoplasm Metastasis;
Phosphorylation*;
Transforming Growth Factor alpha*;
Tyrosine*;
Uterine Cervical Neoplasms*
- From:Korean Journal of Gynecologic Oncology and Colposcopy
2000;11(1):13-23
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: Cadherin/catenin adhesion complex is fundamentally involved in epithelial cancer invasion and metastasis. E-cadherin and EGFR colocalize on the basolateral membrane of epithelial cell and EGF down-regulate E-cadherin expression. In the invasion and metastasis of cancer, E-cadherin expression is decreased and growth factors receptor is overexpressed. The present study was aimed to find the role of E-cadherin, beta-and gamma-catenin, growth factors and its receptors in cervical cancer cell lines. METHODS: The cervical cancer cell cultures were treated with different time duration of EGF 30 ng/ml and TGF-a 10 ng/ml(0, 10 min, 20 min, 30 min, 1 hr, 2 hr, 4 hr, 8 hr, 24 hr). The change in cancer cell morphology and the changes in E-cadherin, beta- and gamma-catenin, EGFR and activated EGFR expression were studied with a western blot analysis and an immunoprecipitation. RESULTS: Through a western blot analysis, E-cadherin 120 kDa band and EGFR 170 kDa band were expressed in CaSki, HT-3 and ME-180 cell line, which showed epithelial contact growth. 1n these 3 cell lines, expression of E-cadherin did not decrease with time dependent manner. after the treatment of EGF and TGF- alpha. The expression of EGFR decreased and activated EGFR expression increased in 30 minutes to 1 hour but decreased subsequently. When the cells treated with EGF, there were no change in beta-and gamma-catenin expression with there dependent manner. The tyrosine phosphorylation of beta-and gamma-catenin increased in 30 minutes to 1 hour but decreased subsequently with activated EGFR. CONCLUSION: This study showed that an activated EGFR which has involved with tyrosine phosphorylation of beta- and gamma-catenin influenced by growth factors rather than expression of E-cadherin, has a role in the invasion and metastasis of the cervical cancer.
