Effect of c-Jun NH2-terminal kinase-mediated p53 expression on neuron autophagy following traumatic brain injury in rats
10.3760/cma.j.issn.0366-6999.2012.11.032
- Author:
Ming-Yan HONG
1
;
Jun-Ling GAO
;
Jian-Zhong CUI
;
Kai-Jie WANG
;
Yan-Xia TIAN
;
Ran LI
;
Hai-Tao WANG
;
Huan WANG
Author Information
1. Department of Surgcry, Hebei Medical University, Shijiazhuang,Hcbei 050017, China
- Keywords:
brain injuries;
autophagy;
c-Jun NH2-terminal kinase;
p53;
damage-regulated autophagy modulator
- From:
Chinese Medical Journal
2012;(11):2019-2024
- CountryChina
- Language:Chinese
-
Abstract:
Background Activation of c-Jun NH2-terminal kinase (JNK) has been implicated in neuron apoptosis as well as autophagy in response to various stressors after traumatic brain injury (TBI).However,the underlying molecular pathway remains unclear.Our study assessed whether JNK-mediated p53 phosphorylation might be an important mechanism for enhancing neuron autophagy in response to TBI.Methods A total of 186 male Sprague-Dawley (SD) rats (300-350 g) were used in this study.By randomized block method rats were randomly divided into four groups:sham-operated (n=46),TBI (n=60),TBI + dimethyl sulfoxide (DMSO) (n=40),and TBI + SP600125 (n=40).JNK was treated with SP600125,a specific JNK inhibitor.JNK,p-P53,Beclin-1,damage-regulated autophagy modulator (DRAM) and p-bcl-2 were evaluated by Western blotting analysis.The cellular localization and expression of Beclin-1 and DRAM was observed by immunofluorescence and immunohistochemistry,and the expression of Beclin-1-Bcl-2/Bcl-xL complexes was evaluated by immunoprecipitation.Multiple-group comparisons were conducted using analysis of variance (ANOVA).P values of less than 0.05 were considered statistically significant.Results It was observed that the expression of JNK,p-P53,Beclin-1,DRAM and p-bcl-2 was increasing after TBI,and the expression of Beclin-1 and DRAM was mainly located in the cytoplasm of neurons.But these were significantly inhibited in SP600125 group compared with sham group and TBI+SP600125 group (P ＜0.05).The expression of Beclin-1-Bcl-2/Bcl-xL complexes was reduced after TBI.Conclusion JNK-mediated p53 phosphorylation might be an important mechanism for enhancing neuron autophagy in response to TBI.
