α-Synuclein immunoreactivity and ultrastructural study of glial cytoplasmic inclusions in multiple system atrophy
10.3760/j.issn:0366-6999.2002.10.013
- VernacularTitle:多系统萎缩胶质细胞包涵体对α-共核蛋白的免疫反应性和超微结构的研究
- Author:
Yin WANG
1
;
Chuanzhen LU
;
Zhurong YE
Author Information
1. 复旦大学
- Keywords:
α-synuclein @ glial cytoplasmic inclusions GCIs ) @ multiple system atrophy (MSA ) pathogenesis
- From:
Chinese Medical Journal
2002;115(10):1491-1495
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the possible pathogenesis of sporadic multiple system atrophy (MSA).Methods The immunoreactivity and ultrastructural features of glial cytoplasmic inclusions (GCIs) in 12autopsy patients with MSA and 4 normal control groups were studied. All regional sections from eachsubject were evaluated with HE staining, KIuver-Barrera (KB), Holzer's, modified Gallyas-Braak's (GB)methods and immunohistochemical staining with α-synuclein and ubiquitin antibodies. Pontine white matterwith abundant GCls from case 1 was examined, using conventional electron microscopy, Gallyas-Braak'selectron microscopy and immunoelectron microscopy.Results The presence of GCls as constantly demonstrated in all MSA patients. Strong α-synucleinimmunoreactivity was observed in all of the ubiquitinated GCls. However, the density of α-synuclein positiveGCIs differed from case to case, and there was no relationship between the density of GCls and age, sex,or MSA subtype. Ultrastructural features indicated that argyrophilic granule-associated filaments of about 25nm in diameter were the predominant constituents of GCls, and the anti α-synuclein antibody selectivelylabeled in these filaments. No GCls and α-synuclein immunoreaction were found in control brain tissues.Conclusions GCI was a pathognomonic change in sporadic MSA patients. Accumulation of α-synuclein inGCIs may occur during the early stags of MSA. Seletcive α-synuclein positive abnormal microtubules inGCls therefore play an important role in the pathogenesis of MSA.
