Low power density microwave radiation induced early changes in rabbit lens epithelial cells

VernacularTitle:受低强度微波辐射后兔晶体上皮细胞的早期改变
Author: Juan YE ¹ ; Ke YAO ; Deqiang LU ; Renyi WU ; Huai JIANG
Author Information

1. Affiliated Second Hospital Medical College
Keywords: microwave; radiation damage; cataractogenesis; lens opacity; epithelial cells
From: Chinese Medical Journal 2001;114(12):1290-1294
CountryChina
Language:Chinese
Abstract: Objective To determine whether low power density microwave radiation can induce irreversible changes in rabbit lens epithelial cells (LECs) and the mechanisms of the changes.Methods One eye of each rabbit was exposed to 5mW/cm2 or 10mW/cm2 power density microwaves for 3 hours, while the contralateral eye served as a control. Annexin Ⅴ-propidium iodide (PI) two-color flow cytometry (FCM) was used to detect the early changes in rabbit lens epithelial cells after radiation. Results Lots of rabbit LECs were in the initial phase of apoptosis in the 5mW/cm2 microwave radiation group. A large number of cells became secondary necrotic cells, and severe damage could be found in the group exposed to 10mW/cm2 microwave radiation. Conclusion Low power densities of microwave radiation (5mW/cm2 and 10mW/cm2) can induce irreversible damage to rabbit LECs. This may be the non-thermal effect of microwave radiation.