OxLDL upregulates growthregulation oncogene α expression in human endotheli al cells
10.3760/j.issn:0366-6999.2001.12.002
- VernacularTitle:氧化低密度脂蛋白功能性调节人内皮细胞的生长调节致癌基因α
- Author:
Zhubin LEI
1
;
Xiaoyan LI
;
Lingbo QING
;
Min WEI
;
Chaoyang WEN
;
Jing XU
Author Information
1. General Hospital of Jinan Military Area
- Keywords:
oxLDL;
chemokine;
GROα;
endothelial cell;
monocyte;
atherosclerosis
- From:
Chinese Medical Journal
2001;114(12):1240-1244
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of oxLDL on CXC chemokine growthregulated oncogene α (GROα) expression in human endothelial cells and the possible functional signific ance of the effect Methods LDL was isolated by sequential ultracentrifugation and oxidized to oxLDL Rever se transcriptionpolymerase chain reaction with GAPDH as internal standard was applied and CXC chemokine GROα mRNA in endothelial ECV304 cells was examined ELISA was used to determine GROα protein expression on ECV304 cell surface and in the medium With static cell adhesion assays, the physiological significa nce of elevated GROα expression was tested Results OxLDL, not LDL, treatment of ECV304 cells significantly induced the expression o f GROα mRNA that was not detectable in untreated cells Induction of expressio n was first evident at 1h, became maximal at 2h, and was substantially decre ased by 4h In a concentration and timedependent manner, oxLDL, and not L DL, induced a significant upregulation of GROα surface expression in ECV304 cel ls that was at a barely detectable level in unstimulated ECV304 cells GROα protein in the medium did not change significantly Exposure of ECV304 cells to 40μg protein /ml oxLDL for 24h resulted in a marked increase in the number of U937 cells bound to ECV304 cells and antibodies to GROα inhibited adhesion Conclusion OxLDL functionally upregulated GROα expression in endothelial cells
