Homocysteine induces expressions of adhesive molecules on leukocytes in whole blood

VernacularTitle:同型半胱氨酸增高血白细胞表面粘附分子的表达
Author: Xuewei GUO ¹ ; Peter Nicholas DUDMAN
Author Information

1. Gansu Province People's Hospital Lanz hou 730000 China
Keywords: homocysteine; adhesive molecules; leukocyt es
From: Chinese Medical Journal 2001;114(12):1235-1239
CountryChina
Language:Chinese
Abstract: Objective To observe whether homocysteine can directly alter the expressions of CD11b, CD18, CD14 and Lselectin on neutrophils, monocytes, and lymphocytes in whole blood from healthy human subjectsMethods Leukocyte surface adhesive molecule expressions were analyzed by immunofluorescence flow cytometry Results Homocysteine at the lowest concentration (20μmol/L) significantly increased surface adhesive molecule expressions of CD11b and CD18 on each cell type and CD14 on monocytes and neutrophils in whole blood These effects were increased at homocysteine concentrations of 200 and 400μmol/L, but at concentrations 1 mmol/L, CD11b/CD18 and CD14 expressions on all types of leukocytes were decreased Lselectin expression was slightly decreased on all cell types in whole blood by homocysteineConclusion Homocysteine alters leukocyte expressions of CD11b/CD18, CD14 and Lselectin on leukocytes, which may be involved into homocysteineinduced leukocyte adhesion and migration