Genotypes and polymorphisms of mutant CCR5-△32,CCR2-64I and SDF1-3' A HIV-1 resistance alleles in indigenous Han Chinese
10.3760/j.issn:0366-6999.2001.11.010
- VernacularTitle:中国汉族人群中HIV抗性的CCR5-△32、CCR2-64和SDF1-3'A等位基因型和多态性研究
- Author:
Fusheng WANG
1
;
Lei JIN
;
Zhouyun LEI
;
Hong SHI
;
Weiguo HONG
;
Dongping XU
;
Jiandong JIANG
;
Yue WANG
;
Bing ZHANG
;
Mingxu LIU
;
Yueqi LI
Author Information
1. 解放军第302医院
- Keywords:
HIV-1;
chemokine receptor 5(CCR5);
polymorphism;
allelic frequency;
mutation
- From:
Chinese Medical Journal
2001;114(11):1162-1166
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the frequencies and polymorphisms of CCR5-△32,CCR2-641 and SDF1-3'A alleles conferring resistance to HIV-1 infection in Chinese population from Han ethnic origin.Methods This cohort was comprised of 1251 subjects(915 men and 336 women)aged 15 -80 years and none was HIV-1 positive.Genotyping of allelic CCR5-△32,CCR2-641 and SDF1-3' A variants was performed using PCR or PCR/RFLP assay,and further confirmed by direct DNA sequencing.Results Our finding shows that the△32 deletion mutation in the CCR5 gene does occur in this population and can be inherited in a Mendelian fashion in indigenous Han Chinese at a very low frequency of 0.00119(n= 1254).The frequencies of mutant CCR2-641 and SDF1-3'A alleles were 0.20023(n = 1251)and 0.2873(n = 893),in this population,which are higher than those found in American Caucasians.Furthermore the polymorphisms of CCR2-641 and SDF1-3' A alleles in the Han Chinese population were different from those in American Caucasians.Statistical analysis showed that the genotype distribution of CCR5-△32,CCR2-641 and SDF1-3' A alleles was in equilibrium according to the Hardy-Weinberg equation.Conclusion The CCR5-△32 mutation may not be a major resistant factor against HIV-1 infection in indigenous Han Chinese.The significance of higher frequencies of CCR2-641 and SDF1-3' A alleles (0.20023 and 0.2791)in the Han population remains to be clarified in HIV-1-positive carriers and AIDS patients.
