Intravascular local gene transfer mediated by protein-coated metallic stent
10.3760/j.issn:0366-6999.2001.10.009
- VernacularTitle:金属支架蛋白涂层所介导的血管内局部转基因
- Author:
JQ YUAN
1
;
RL GAO
;
RW SHI
;
LF SONG
;
J TANG
;
YL LI
;
CJ TANG
;
L MENG
;
WM YUAN
;
ZJ CHEN
Author Information
1. Cardiovascular Institute Fu Wai Hospital
- From:
Chinese Medical Journal
2001;114(10):1043-1045
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the feasibility, efficiency and selectivity of adenovirus-mediated gene transfer to local arterial wall by protein-coated metallic stent. Methods A replication-defective recombinant adenovirus carrying the Lac Z reporter gene for nuclear specific β-galactosidase (Ad-βgal) was used in this study. The coating for metallic stent was made by immersing it in a gelatin solution containing crosslinker. The coated stents were mounted on a 4.0 or 3.0 mm percutaneous transluminal coronary angioplasty (PTCA) balloon and submersed into a high-titer Ad-βgal viral stock (2 × 1010 pfu/ml) for 3 min, and then implanted into the carotid artedes in 4 mini-swines and into the left anterior descending branch of the coronary artery in 2 mini-swines via 8F large lumen guiding catheters. The animals were sacrificed 7 (n=4), 14 (n = 1) and 21 (n = 1 ) days after implantation, respectively. The β-galactosidase expression was assessed by X-gal staining. Results The results showed that the expression of transgene was detected in all animal. In 1 of carotid artery with an intact intima, the β-gal expression was limited to endothelial cells. In vessels with denuded endothelium, gene expression was found in the sub-intima, media and adventitia. The transfection efficiency of medial smooth muscle cells was 38.6%. In 2 animals sacrificed 7 days after transfection, a microscopic examination of X-gal-stained samples did not show evidence of transfection in remote organs and arterial segments adjacent to the treated arterial site. Conclusions Adenovirus-mediated arterial gene transfer to endothelial, smooth muscle cells and adventitia by protein-coated metallic stent is feasible. The transfection efficiency is higher. The coated stent may act as a good carrier of adenovirus-mediated gene transfer and have a potential to prevent restenosis following PTCA.
