Immune privilege induced by cotransplantation of islet and allogeneic testicular cells
10.3760/j.issn:0366-6999.2001.10.005
- VernacularTitle:胰岛与FasL+睾丸细胞共移植产生免疫豁免
- Author:
P LAN
1
;
WH ZHAN
;
JP WANG
;
XJ WU
;
LN YAN
;
LJ XIAO
Author Information
1. First Affiliated Hospital Sun Yat-sen University of Medical Sciences
- From:
Chinese Medical Journal
2001;114(10):1026-1029
- CountryChina
- Language:Chinese
-
Abstract:
Objective To induce islet allograft long-term survival through cotransplantation of islet cells with sertoli cells. Methods Testicular sertoli cells were prepared by digestion with collagenase, trypsin and DNase, and were cultured for 48 hours. Collagenase digested and Ficoll purified donor (Wistar rat) islets were cotransplanted with allogeneic sertoli cells in the absence of systemic immunosuppression. Terminal leoxynucleotidyl transferase-mediated X-dUTP nick-end labeling (TUNEL) was used to label apoptosis of lymphocytes surrounding the islet graft. Results Cotransplantation of islets and 1 × 107 sertoli cells reversed the diabetic state for more than 60 days in 100% (6/6) of the chemically diabetic Sprague Dawley rats. Grafts consisting of islets alone or islets plus 1 × 105 sertoli cells survived only for 5 - 6 days. Apoptosis of lymphocytes surrounding the islets was quite clear. Conclusion Cotransplantation of islets with FasL+ sertoli cells induces local immune privilege and allows long-term graft survival without systemic immunosuppression.
