Cell cycle analysis by cyclin E+A/DNA multiparameter flow cytometry in exponential growth MOLT-4 cells

VernacularTitle:对MOLT-4细胞用cyclin E+A/DNA流式细胞术分析 - 细胞周期六分法的建立
From: Chinese Medical Journal 2001;114(9):967-971
CountryChina
Language:Chinese
Abstract: Objective　To establish a new method for analyzing the cell cycle in tumor which is a kind of cell cycle disease. Methods　Mixtures of cyclin E and cyclin A antibodies were incubated with fixed MOLT-4 cells, and measured by flow cytometry. Results　We developed a cyclin E+A/DNA flow cytometry analysis method, which may distinguish G0, early G1, late G1, S, G2 and M phase cells, rather than three phases in the DNA content histogram. Conclusion　Cyclin E+A/DNA multiparameter flow cytometry can simultaneously differentiate in the same sample six cell groups: G0, early G1, late G1, S, G2 and M phase cells. It performed better than any other cell cycle analysis methods that we have used and has a definite cell biology foundation.