Histochemical study of transmyocardial laser channels in dogs
10.3760/j.issn:0366-6999.2001.09.002
- VernacularTitle:激光心肌血运重建术后孔道的组织化学观察
- From:
Chinese Medical Journal
2001;114(9):902-905
- CountryChina
- Language:Chinese
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Abstract:
Objective Laser-induced myocardial injury was investigated in a canine transmyocardial laser revascularization model. Methods A total of 23 channels were created in the left ventricles of 5 animals with a CO2 laser. Four animals were sacrificed immediately and one at week 2. Lactate dehydrogenase and succinate dehydrogenase in channel regions were examined with a Nitro-BT method staining. Results Immediately after the procedure, laser channels and surrounding injured myocardium could be stratified from the center to the border into 5 concentric zones, i.e., vaporized zone, carbonized zone, enzyme-disappeared zone, enzyme-decreased zone and enzyme-concentrated zone. Two weeks later, the phenomenon disappeared. Conclusions Immediately after transmyocardial revascularization, there is a ring of enzyme-concentrated zone at the outermost region of injured myocardium. Two weeks later, enzyme activities of reversibly injured myocytes appear to have recovered.
