Confirmation of susceptibility gene loci on chromosome 1 in Northern China Han families with type 2 diabetes
10.3760/j.issn:0366-6999.2001.08.023
- VernacularTitle:中国北方汉族人群Ⅱ型糖尿病家系1号染色体易感基因位点定位的确证
- Author:
WeiNan DU
1
;
Hongxia SUN
;
MM XIONG
;
JIN ZUO
;
Fude FANG
;
Hong WANG
;
Qi SUN
;
BoQin QIANG
;
Yan SHEN
;
ZJ YAO
;
Jun GU
;
Wei HUANG
;
Zhu CHEN
;
XioFeng HUA
;
Wei GAO
Author Information
1. Chinese Academy of Medical Sciences & Peking Union Medical College
- Keywords:
type 2 diabetes;
microsatellte markers;
genome screening;
susceptibility genes;
gene mapping
- From:
Chinese Medical Journal
2001;114(8):876-878
- CountryChina
- Language:Chinese
-
Abstract:
Objective To confirm previous effort to identify type 2 diabetes susceptibility genes in a Northern Chinese population by conducting a new genome scan with both an increased number of type 2 diabetes families and a new set of microsatellite markers within the previously localized regions. Methods A genome scan method was applied. After multiplexed PCR, electrophoreses, genescan and genotyping analysis, we obtained size information for all loci , and then a further study was done by both parametric and non-parametric linkage analysis to investigate the P values and Z values of these loci. Results We surveyed 34 microsatellite markers which distributed within 5 regions along chromosome 1, and a total of 12?000 genotypes were screened. Evidence of linkage with diabetes was identified for 8 of the 34 loci. All P values of the 8 loci were lower than 0.05, and the highest Z value was 2.17. A very interesting finding is that all 5 markers at the p- terminal 1p36.3-1p36.23 region, spanning a long range of 16.9?cM, were identified to have a low P value of less than 0.05, which suggests that this region may contain multiple susceptibility genes. Regions 4 and 5 also confirmed the previous findings, and we narrowed these two regions to a 2.7?cM and 2.5?cM regions, respectively. Conclusions We further confirmed the results gained in the previous genome-wide scan using an increased number of NIDDM families and a new set of microsatellite markers lying within the initially localized regions. The fact that all 5 loci at the p- terminal region displayed a low P value of less than 0.05 suggests that more than 1 susceptibility gene may reside in this region.
