Detection of human herpesvirus 8 DNA in acute leukemia patients

VernacularTitle:人类疱疹病毒8型DNA在急性白血病患者中的检测分析
Author: Minhui WANG ¹ ; Yuhua SONG ; XiaoTong MA ; MingZhi HAN ; YingChang BI ; YongMin LIN ; Ge LI ; Kefu WU ; GuiFan MU
Author Information

1. Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College
Keywords: human herpesvirus 8; acute leukemia; acute myelogenous leukemia; polymerase chain reaction
From: Chinese Medical Journal 2001;114(8):873-875
CountryChina
Language:Chinese
Abstract: Objective　To determine the prevalence of human herpesvirus 8 (HHV-8) DNA in acute leukemia (AL) patients. Methods　The presence of HHV-8 DNA sequences in peripheral blood mononuclear cells (PBMC) and bone marrow mononuclear cells (BMMC) from 50 AL patients was examined using polymerase chain reaction (PCR). Nine human hematopoietic cell lines and PBMC from 30 normal donors were also included. Results　HHV-8 DNA sequences were detected in one case of acute myelogenous leukemia (AML). The specimens from the bone marrow aspirate, peripheral blood and serum of this patient were all positive. None of the normal donors and human hematopoietic cell lines showed evidence of HHV-8 DNA. Conclusion　The results suggest that the prevalence of HHV-8 is low in AL in China.