Identification and function of glucose transporter 1 in human mesangial cells
10.3760/j.issn:0366-6999.2001.08.010
- VernacularTitle:人肾小球系膜细胞葡萄糖转运蛋白的鉴定及功能研究
- Author:
Yingjian LI
1
;
ZhiHong LIU
;
Dong LIU
;
Jing ZHANG
;
ZhaoHong CHENG
;
LeiShi LI
Author Information
1. 南京大学附属南京军区南京总医院(金陵医院)
- Keywords:
glucose transporter 1;
mesangial cells;
diabetic nephropathy
- From:
Chinese Medical Journal
2001;114(8):824-828
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of glucose transporter 1 (GLUT1) in the glucose uptake of glomerular mesangial cells. Methods Cultured human glomerular mesangial cells were used. The expressions of glucose transporter 1 mRNA and protein were detected with reverse transcription-polymerase chain reaction (RT-PCR), flow cytometry and immunofluorescence staining. Glucose uptake was determined with 2-deoxy-［3H］-D-glucose uptake assay. Results The expressions of GLUT1 mRNA and proteins were detected in human mesangial cells. Glucose uptake and its kinetics assay showed that GLUT1 is a functional glucose transporter in cultured human mesangial cells, and that its function could be inhibited by the specific inhibitor, Phloretin. Conclusion GLUT1 is the predominant glucose transporter in human mesangial cells, which has the kinetic characteristics of high affinity and low capacity for D-glucose. This suggests that in order for mesangial cells to take up excessive quantities of glucose, as in diabetes, changes in glucose transporter expression, translocation or activity may be required.
