Epitope mapping on Wegener's granulomatosis autoantigen proteinase 3
10.3760/j.issn:0366-6999.2001.07.022
- VernacularTitle:韦格纳肉芽肿病自身抗原蛋白酶3的抗原决定簇研究
- Author:
Lockwood Martin CHRISTOPHER
1
;
Lockwood Martin CHRISTOPHER
Author Information
1. University of Cambridge School of Clinical Medicine Addenbrooke's Hospital
- Keywords:
vasculitis;
Wegener's granulomatosis;
proteinase 3;
anti-neutrophil cytoplasmic autoantibodies;
epitope
- From:
Chinese Medical Journal
2001;114(7):760-763
- CountryChina
- Language:Chinese
-
Abstract:
Abstract:Objective To map the epitopes on Wegener's granulomatosis autoantigen proteinase 3.Methods Antigenicity of proteinase 3 was studied with Western blot analysis in which proteinase 3 was prepared under reducing and non-reducing conditions. Two anti-proteinase 3 monoclonal antibodies, HZ1F12 and HZ1H3, were used to inhibit each other and to inhibit 22 anti-proteinase 3 positive sera from patients with Wegener's granulomatosis in competitive inhibition enzyme-linked immunosorbent assays (ELISA) and Western blot analysis. Results All monoclonal antibodies and patient sera recognized proteinase 3 under non-reducing conditions in Western blot analysis. HZ1F12 was inhibited 74% by HZ1H3. 10/22 (46%) sera were completely or partially inhibited by HZ1F12; 9/22 (41%) sera were partially inhibited by HZ1H3; and 6/22 (27%) were inhibited by both monoclonal antibodies. In inhibition Western blot analysis, the binding of patient sera to proteinase 3 could be inhibited by HZ1F12. Conclusions The epitopes of Wegener's granulomatosis autoantigen were conformational. Anti-proteinase 3 monoclonal HZ1F12 and HZ1H3 recognized similar or overlapping epitopes on the proteinase 3 molecule. Epitopes of proteinase 3 recognized by anti-proteinase 3 positive sera were not restricted.
