In-vitro cultivation of normal human oral keratinocytes
- VernacularTitle:体外连续培养人口腔粘膜角化细胞
- Author:
Zengtong ZHOU
1
;
Haiwen ZHOU
;
Qingxin SHANG
;
Yilin CAO
Author Information
1. Shanghai Second Medical University
- Keywords:
cell culture;
oral keratinocytes/epithelial cells;
morphology;
cytokeratin
- From:
Chinese Medical Journal
2001;114(7):731-734
- CountryChina
- Language:Chinese
-
Abstract:
Abstract:Objective To establish a method for culturing normal human oral keratinocytes.Methods Specimens obtained from healthy humans undergoing oral surgery were dissociated into single cell suspensions by dispase and trypsin. The cells were grown in serum-free medium. Morphological characteristics were studied under light microscope and electron microscope. Cytokeratins were shown by immunohistochemistry.Results Cells could be maintained in culture up to 4-5 passages or 30-50 days. Electron microscope revealed that there were desmosomes and tonofibrils in the oral keratinocytes. The cells showed positive staining for cytokeratin antibody. Conclusion Human oral keratinocytes have been successfully grown in serial culture.
