Detection of p53 gene mutations in sputum sample and their implications in the early diagnosis of lung cancer in suspicious patients
- VernacularTitle:痰标本检测p53基因突变及其在肺癌早期临床诊断中的意义
- Author:
Binchao WANG
1
;
Longyun LI
;
Lianchang YAO
;
Lihua LIU
;
Yuanjue ZHU
Author Information
1. Peking Union Medical College Hospital
- Keywords:
lung neoplasms;
p53 gene;
diagnosis;
PCR-SSCP-silver stain
- From:
Chinese Medical Journal
2001;114(7):694-697
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To evaluate the value of detecting p53 gene point mutations in sputum samples and its validity and reliability as a surveillance index in the early diagnosis of lung cancer in suspicious patients.Methods Sputum samples were collected from 54 cases identified as lung cancer and 114 cases identified as pulmonary benign disease. The polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was performed for the detection of point mutations at exons 5-8 of the p53 gene, and sputum smears were also used for each sample. Conclusion Detection of p53 gene alterations in sputum samples by PCR-SSCP-silver stain can be used as a follow-up surveillance index for the early diagnosis of lung cancer in suspicious patients.
