Chimera formation of platelet GP Ⅱb Bak a/b by intrauterine transplantation of fetal liver stem cells
10.3760/j.issn:0366-6999.2001.07.002
- VernacularTitle:宫内胎肝细胞移植形成血小板GPⅡb Bak a/b嵌合体的研究
- Author:
Fangping CHEN
1
;
Qinzhi XIE
;
Zaifu JIAN
;
Xinhua ZHANG
;
Lingna ZHANG
;
Xinhua WU
Author Information
1. Xiang Ya Hospital Central South University
- Keywords:
platelet GPⅡb Bak a/b;
artificial chimera;
human fetal liver stem cells;
intrauterine transplantation
- From:
Chinese Medical Journal
2001;114(7):676-679
- CountryChina
- Language:Chinese
-
Abstract:
Abstract:Objective To investigate whether artificial heterozygous chimeras of platelets can be established by intrauterine transplantation of fetal liver stem cells and evaluate its potential use for the treatment of Glanzmann thrombasthenia.Methods Platelet glycoprotein (GP) Ⅱb Bak a/b (or GPⅡb Ⅰle843Ser) was used as a genetic marker. A homozygous 16-week-old Bak a/a fetus (as donor) and a homozygous 16.5-week-old Bak b/b fetus (as recipient) were screened from 42 pregnant women hospitalized for abortion. PCR with allele specific primers and FOK Ⅰ digestion based on PCR products were used. Aborted donor fetal liver cell suspensions were prepared and intrauterine transplantation was carried out by infusion of 4?ml fetal liver cells (22×105) into the recipient umbilical vein under ultrasonic visualization.Results At gestation termination (abortion), 21 days after transplantation, chimera GPⅡb Bak a/b of the recipient were detected by FOK 1 digestion based on PCR from DNA and RT-PCR from platelet RNA. Conclusion Intrauterine transplantation of fetal liver cell may provide an effective way for curing GT or other inherited diseases.
