Increased expression of Hsp70 and co-localization with nuclear protein in cells infected with the Hantaan virus
10.3760/j.issn:0366-6999.2001.05.022
- VernacularTitle:汉滩病毒感染细胞中Hsp70和NP的高表达及共定位
- Author:
Ling YE
1
;
Yanfang LIU
;
Shoujing YANG
;
Wenjun LIAO
;
Chunmei WANG
Author Information
1. The Fourth Military Medical University
- From:
Chinese Medical Journal
2001;114(5):535-539
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Hantaan virus infection on the expression of stress genes. Methods Techniques of virus infection, Western blot, immunohistochemistry, dual-immunofluorecsence staining, laser scanning confocal microscopy, RNA dot blot and in situ hybridization were used.Results Expression of heat shock protein 70 (Hsp70) was observed in cells infected with HTV as well as the translocation of Hsp70 from cytoplasm to nucleoli following virus infection. The variable distribution of Hsp70 was related to the various time after infection. Double-label indirect immunofluorescence of nuclear protein (NP) and Hsp70 in infected cells demonstrated co-localization of these proteins in the cytoplasm. Conclusion Overexpression of Hsp70 can be induced directly by Hantaan virus, which may be associated with virus protein assembly. The Hantaan virus proteins co-localize with, and possibly form a physical complex with cellular Hsp70 in infected cells.
