Molecular cloning and characterization of NAG-7: a novel gene downregulated in human nasopharyngeal carcinoma
10.3760/j.issn:0366-6999.2001.05.021
- VernacularTitle:一个在鼻咽癌中表达下调的新基因NAG-7的克隆及其特征分析
- Author:
Yi XIE
1
;
Lianghua BIN
;
Jianbo YANG
;
Zhonghua LI
;
Ying YU
;
Xiaohui ZHANG
;
Li CAO
;
Guiyuan LI
Author Information
1. 湖南大学
- From:
Chinese Medical Journal
2001;114(5):530-534
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify novel tumor suppressor genes at chromosome 3p24-26 in human nasopharyngeal carcinoma (NPC).Methods Twenty epithelial-derived expressed sequence tags (EST) were selected from chromosome 3p24-26. RT-PCR and Northern blot were used to detect the expression of the ESTs in NPC cell line, HNE-1, and primary cultures of normal nasopharyngeal epithelial cells. One EST, which was substantially downregulated in the HNE-1 cell line, was detected in 19 NPC biopsy samples, cDNA library screening was used to get its full sequence and the sequence of this novel gene was analyzed.Results A novel gene located at chromosome 3p25.3 was obtained and named NAG-7. It was downregulated in 26.3％ (5/19) of NPC biopsy samples. Its 1677 bp full length cDNA had a potential open reading frame predicting a 94 amino acid protein with a molecular weight of 11023.87 Dalton. Analysis of the NAG-7 gene showed that it was a transmembrane protein containing a protein kinase C phosphorylation site and a myristyl site. It has no significant homology to any reported genes in the database of GenBank. Conclusion NAG-7 is a novel gene downregulated in NPC, suggesting that it may be involved in the development of NPC.
