Relationship between c-fos gene expression and delayed neuronal death in rat neonatal hippocampus following hypoxic-ischemic insult
10.3760/j.issn:0366-6999.2001.05.019
- VernacularTitle:缺氧缺血后新生鼠脑c-fos表达与海马迟发性神经元死亡
- Author:
Li JIANG
1
;
Yanjie DING
;
Yunzhen TANG
Author Information
1. Affiliated Hospital Nanjing Railway Medical College
- From:
Chinese Medical Journal
2001;114(5):520-523
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of perinatal hypoxia-ischemia encephalopathy, we studied the expression of the c-fos gene and its relationship with delayed neuronal death in a rat model.Methods Cerebral hypoxia-ischemia was produced in 7-day-old SD rats using the Rice model, Reverse transcription PCR (RT-PCR), immunohistochemistry and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) were used to detect the expression of c-fos gene and cell apoptosis in the hippocampus.Results The selective expression of c-fos and delayed cell apoptosis were observed in the hypoxiaischemia hippocampus. Expression of the c-fos gene was seen in the CA4 and cingulate sulcus neurons, and apoptosis was observed in the CA1 neurons.Conclusion Transient expression of the c-fos gene may induce cerebral cell apoptosis, and may have complex relations with delayed cell death.
