Stable clonal expansion of the T-cell receptor Vβ6, Vβ17 and Vβ19 T cells in a cGVHD case using genescan analysis
10.3760/j.issn:0366-6999.2001.05.011
- VernacularTitle:基因扫描分析一例cGVHD病人稳定性克隆性扩增的TCR Vβ6，Vβ17和Vβ19T细胞
- Author:
Xin DU
1
;
Minhua XU
;
Tao ZHANG
;
Yangqiu LI
;
Lijian YANG
;
Shaohua CHEN
Author Information
1. 暨南大学
- From:
Chinese Medical Journal
2001;114(5):489-492
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution and clonality of the T-cell receptor (TCR) Vβ repertoire in chronic graft versus host disease (cGVHD).Methods The complementarity determining region 3 (CDR3) of the TCRβ gene with 24 variable regions was amplified in peripheral blood mononuclear cells drawn from one cGVHD patient after allogenic bone marrow transplantation (allo-BMT) 35, 39, 43 or 45 months respectively, using RT-PCR, to observe the expression of TCR Vβ repertoire T cells. The PCR products were further analyzed by genescan to evaluate clonality of T cells. Ressults Fourteen or 16 TCR Vβ subfamily T ceils were detected in each sample of cGVHD case. Oligoclonal T cells were identified in TCR Vβ 6, 16, 17, 19 and 21 subfamilies. The stable clonal T cells in all samples were identified in Vβ6, Vβ17 and Vβ21 subfamilies.Conclusion Skewing distribution and stable clonal expansion of T cells can be found in cGVHD cases and it may be related to the initiation of cGVHD.
