Growth inhibition of interleukin-2 receptor gene-transduced peripheral blood lymphocytes on human ovarian cancer cells
10.3760/j.issn:0366-6999.2001.03.017
- VernacularTitle:IL-2R基因转染PBL对人卵巢癌细胞生长抑制的影响
- Author:
Dafeng YE
1
;
Xing XIE
;
Weiguo LU
;
Huaizeng CHEN
;
Bei CHENG
Author Information
1. 浙江大学附属妇产科医院
- Keywords:
ovarian neoplasm lymphocyte interleukin-2 interleukin-2 receptor gene therapy
- From:
Chinese Medical Journal
2001;114(3):303-307
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the growth inhibition of interleukin-2 receptor (IL-2R) gene-transduced peripheral blood lymphocytes (PBLs) on human ovarian cancer cells. Methods Interleukin-2 (IL-2) and IL-2R genes were transfected into human ovarian cancer cell line 3AO and PBLs, respectively, using the same Fugene vector. Twenty-four hours later transfected and nontransfected PBLs were cocultured with transfected and nontransfected 3AO for 48 hours. Cytotoxity of PBLs on 3AO was detected by the MTT assay. Results The morphology of IL-2-transduced 3AO and IL-2R-transduced PBLs remained unchanged. 3AO cells could be transfected with the IL-2 gene and expressed IL-2 mRNA, and PBLs could be transfected with the IL-2R gene and expressed IL-2R mRNA. IL-2 transduced 3AO cells enhanced their response to the cytotoxity of PBLs. Furthermore, growth inhibition of PBLs to 3AO cells increased significantly when the IL-2R was transfected into PBLs and when the IL-2 gene was transfected into 3AO cells and the two were combined. Conclusions IL-2R gene transduced PBLs are able to enhance their cytotoxity on IL-2 gene transduced ovarian cancer cells. This method may be a new way to investigate IL-2 gene therapy for ovarian cancer.
