Modulation of BmKAS-1 and BmK1-3-2 to sodium channel in rat dorsal root ganglion neurons

VernacularTitle:BmKAS-1和BmK1-3-2对大鼠背根神经节神经元钠电流的影响
Author: Hang XIAO ¹ ; Xia MAO ; Zhiyong TAN ; Yun SHI ; Zhiqi ZHAO ; Yonghua JI
Author Information

1. Nanjing Medical University
Keywords: patch-clamp BmKAS-1 BmK1-3-2 dorsal root ganglion sodium channel
From: Chinese Medical Journal 2001;114(3):253-256
CountryChina
Language:Chinese
Abstract: Objective　To investigate what effects BmKAS-1 (a polypeptide purified from the Chinese scorpion Buthus martensi Karsch ［BmK］ and named as BmK activator of skeletal-muscle ryanodine receptor) and its upstream mixture BmK1-3-2 have on Na+ channels in dorsal root ganglion (DRG) small diameter neurons. Methods　The whole-cell patch-clamp technique was used to investigate the effects of BmKAS-1 and BmK1-3-2 on Na+ current in rat small diameter DRG neurons. Results　About 50% peak Na+ current was suppressed by 10*!μg/ml of BmK1-3-2. 1.62*!μg/ml of BmKAS-1 also blocked 50% peak Na+ current, and there was an obvious dose-dependent relationship. Conclusion　Both BmK1-3-2 and BmKAS-1 have a blocking effect on Na+ channels, and this may one of the mechanisms for the analgetic effect of BmK1-3-2 and BmKAS-1.