Interferon-γ inhibits in situ expression of PDGF-β mRNA by smooth muscle cells in injured rabbit arteries after transluminal balloon angioplasty

VernacularTitle:γ干扰素抑制兔平滑肌细胞PDGF-β mRNA原位表达和球囊扩张术后再狭窄
Author: Jun JI ¹ ; Lüsheng SI ; Weihua FANG ; Wenping LING
Author Information

1. Shenzhen Sunyixian Cardiovascular Hospital
Keywords: restenosis; smooth muscle cells; proliferating cell nuclear antigen; PDGF-β mRNA; interferon-gamma
From: Chinese Medical Journal 2001;114(2):139-142
CountryChina
Language:Chinese
Abstract: Objective　To elucidate the mechanism of interferon-gamma (IFN-γ) to inhibit the restenosis after successful percutaneous transluminal angioplasty (PTA). Methods　A rabbit vascular restenotic model was constructed and the proliferation of intimal smooth muscle cells (SMCs) were observed by monitoring their expression of proliferating cell nuclear antigen (PCNA) and platelet-derived growth factor β chain mRNA (PDGF-β mRNA) at the indicated time points. Results　IFN-γ could significantly inhibit the expression of PCNA by intimal SMCs one week after denudation, when counting 200 intimal cells for PCNA-positive reactions with an inhibitory rate of 88.50% (P<0.001). IFN-γ could downregulate in situ expression of PDGF-β mRNA by these cells as we calculated the average number of PDGF-β mRNA positive cells per square millimetre area at ×400 magnification with reduced rates of 86.85% in 1 week group (P<0.001), of 93.66% in 2 week group (P<0.001) and of 52.92% in 4 week group (0.02