Endocytic routes of exogenous antigen in murine dendritic cells and macrophage
- VernacularTitle:树突状细胞和巨噬细胞对外源性抗原内吞路径的比较研究
- Author:
Zhifang XIE
1
;
Xuetao CAO
;
Weiping ZHANG
;
Xuting YE
;
Bingxue YU
;
Zun ZHENG
Author Information
1. Department of CellBiology Second Military Medical University
- Keywords:
dendritic cells;
macrophage;
major histocompatibility antigens
- From:
Chinese Medical Journal
2001;114(1):93-96
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the endocytic routes of exogenous antigen between murine dendritic cells (DCs) and macrophages (Mφs). Methods Murine bone marrow-derived DCs and peritoneal Mφs were pulsed with horseradish peroxidase (HRP)-5 nm colloidal gold for 10 minutes, then grouped and chased for 0-120 minutes in culture medium. Intracellular distribution of 5 nm colloidal gold was explored by means of the cellular enzymatic-chemistry of acid phosphatase and MHC Ⅱ immuno-cytochemistry under electron microscope. Results After 10 minutes of pulse with HRP-5 nm colloidal gold and 30 minutes of chase, most HRP-5 nm gold particles internalized by DCs entered into MHC class Ⅱ compartments (MⅡCs), and a small portion entered into acid phosphatase-positive lysosomes. In contrast to DCs, most Mφs lysosomes were accessed by HRP-5 nm gold particles, and a small portion of HRP-5 nm gold particles entered into MⅡCs. After 60 minutes of chase, 5 nm gold particles could hardly be seen within Mφs, whereas most 5 nm gold particles were still retained in DCs. Conclusions The endocytic route of exogenous antigen in DCs seems to be different from that in Mφs. Antigens taken by Mφs mainly enter into lysosomes within 30 minutes. In the case of DCs, most internalized antigens enter into MⅡCs, which may be related to their unique antigen-presenting function . In addition, Mφs seem to have more powerful capacity to scavenge exogenous antigen than DCs.
