Identification of metastasis associated gene G3BP by differential display in human cancer cell sublines with different metastatic potentials G3BP as highly expressed in non-metastatic
10.3760/j.issn:0366-6999.2001.01.007
- VernacularTitle:应用差异显示技术从高低转移癌系克隆出肿瘤转移相关基因G3BP
- Author:
Yuxin LIU
1
;
Jie ZHENG
;
Weigang FANG
;
Jiangfeng YOU
;
Jieliang WANG
;
Xianglin CUI
;
Bingquan WU
Author Information
1. 北京大学
- Keywords:
lung cancer;
cancer metastasis;
G3BP
- From:
Chinese Medical Journal
2001;114(1):35-38
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate genes involved in cancer metastasis, mRNA differential display was used to compare the levels of gene expression in two cell sublines derived from human giant cell carcinoma of lung (PG) which had different metastatic potentials. Methods Using in vivo tumorigenicity and a spontaneous metastasis assay in nude mice, two sublines (BE1, LH7) from human giant cell carcinoma of lung (PG) with different metastatic potentials were isolated and characterized. mRNA differential display was used to compare the levels of gene expression between them and the obtained results were confirmed by Northern hybridization. Results One differentially expressed band was nearly identical (99% homology) to Ras-GTPase-Activating protein SH3 domain binding protein (G3BP). G3BP displayed a strong expression in LH7 (non-metastatic in recipient nude mice) and a very weak expression in BE1 (100% metastatic frequency). The same different expression level of G3BP was detected in Northern hybridization with another panel of cell sublines with different metastatic potentials (established in our lab) derived from human prostate carcinoma cell line PC-3M. Conclusion Our results indicate that G3BP was implicated in cancer metastasis because of its differential expressions in the two panels of cell sublines with different metastatic potentials.
