Comparison between homologies of E2/NS1 gene from genotype Ⅲ Chinese isolates of hepatitis C virus and that from reported isolates
- Author:
Chaodong WU
1
;
Qimin TAO
Author Information
1. 北京医科大学附属人民医院
- From:
Chinese Medical Journal
1998;111(9):807-809
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To sequence E2/NS1 gene from genotype Ⅲ Chinese isolates of hepatitis C virus (HCV) and analyze homology corresponding to the region of the reported isolates.Methods E2/NS1gene derived from genotype Ⅲ Chinese isolates of HCV was amplified by reverse transcripase-polymerase chain reaction (RT-PCR) and cloned into vector pcDNA3. Dideoxy chain termination methods were used to sequence E2/NS1 gene. Results E2/NS1 gene derived from genotype Ⅲ Chinese isolates of HCV was cloned for the first time and named HC-W14. Identity of HC-W14 in nucleotide and putative amino acid to those of genotype Ⅲ Japanese isolates of HCV were 88.37% and 89.29% respectively. Homology to that of non-type Ⅲ isolates was relatively low.Conclusions High variation existed in E2/NS1 region between genotype Ⅲ and Ⅱ Chinese isolates of hepatitis C virus. The variability of E2/NS1 gene should be taken into account in the development of vaccine against HCV in China.
