Herbimycin A enhances apoptotic effect of chemotherapeutic drugs on K562 cells
- Author:
Hanyun REN
1
;
Shanshan CHEN
;
Daopei LU
Author Information
1. 北京医科大学附属人民医院
- From:
Chinese Medical Journal
1998;111(8):678-681
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To explore the anti-apoptotic mechanism and the effective apoptosis-inducing method in chronic myelogenous leukemia (CML) cells.Methods K562 cell line was used to observe the effect of combination of herbimycin A (HMA), a tyrosine kinase inhibitor, and chemotherapeutic agents on the induction of apoptosis.Results HMA or chemotherapeutic agents could inhibit the proliferation but not significantly induce apoptosis of K562 cells. However, HMA significantly enhanced apoptosis when combined with chemotherapeutic agents. Addition of sulfhydryl compound to the cultures to conjugate HMA completely abrogated this enhancing effect on K562 cells.Conclusions HMA increases the sensitivity of CML cells to chemotherapeutic agents by inactivating tyrosine kinase activity. It is promising that combination of HMA with conventional chemotherapeutic drugs may be a new strategy in the treatment of CML.
