Factors affecting release of adrenomedullin and adrenotensin from the aortae of rats
- Author:
Lan ZHOU
1
;
Zongyin QIU
;
Chaoshu TANG
;
Yuntao ZHAO
Author Information
1. 重庆医科大学
- From:
Chinese Medical Journal
1998;(6):541-541
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the release regulation of adrenomedullin (ADM) and adrenotensin (ADT).Methods Incubation or perfusion of the isolated aortae of rats for 1 hr with different factors and radioimmunoassay for ADM and ADT in the media.69%-300% in a dose-dependent manner. One and 10 nmol/L AⅡ and ET-1 had no obvious effects on ADT release. 100 nmol/L AⅡ and ET-1 increased ADT release by 128% and 117%, respectively. The ratios of ADM/ADT of the above-mentioned groups were increased by 36%-111%. 10 nmol/L epinephrine and calcitonin gene related peptide (CGRP) augmented release of ADM and ADT by 101% and 87% and decreased their release by42% and 42%, respectively. However, the ratios of ADM/ADT of the two groups were unchanged. Hypoxia had no effect on ADM release, but it inhibited ADT release by 43%. The ratio of ADM/ADT was increased by 87%. 1 μg/ml lipopolysaccharide (LPS) had no effect on release of ADM and ADT, but 5 μg/ml and 10 μg/ml LPS increased ADM release by 177% and 239%. They increased ADT release by 39% and 74% and ADM/ADT by 97% and 98%, respectively.*h-1, 0.252±0.060 pmol/gw*h-1 and 0.844±0.115, respectively. One, 10 and 100 nmol/L angiotensin Ⅱ (AⅡ) and endothelin-1 (ET-1) increased ADM release by Results The control values of release of ADM and ADT and ADM/ADT in incubation groups were 0.210±0.038 pmol/gw23% and 129%, respectively, but did not change the ratio of ADM/ADT. Decreased flow rate had no effect on their release. P values of the above-mentioned percentages were lower than 0.05 or 0.01.*h-1, 0.282±0.068 pmol/gw*h-1 and 0.835±0.192, respectively. Increased flow rate augmented release of ADM and ADT by 1The control values of release of ADM and ADT and ADM/ADT in perfusion groups were 0.226±0.025 pmol/gwConclusion AⅡ, ET-1, epinephrine, LPS, CGRP, flow rate and oxygen supply participate in the release regulation of ADM and ADT from the aortae of the rats in different manners and degrees.
