Endothelium-dependent relaxation of canine pulmonary artery endothelium after prolonged preservation
- Author:
Yen CHU
1
;
Jing Pyng LIN
;
Chau-Hsiung CHANG
Author Information
1. Chang Gung Memorial Hospital, Chang Gung Medical College
- From:
Chinese Medical Journal
1998;111(4):330-333
- CountryChina
- Language:Chinese
-
Abstract:
Objective Experiments were designed to investigate the effect of Euro-Collins (EC) solution and University of Wisconsin (UW) solution on function of pulmonary arterial endothelium.Methods Third order canine pulmonary artery segments were preserved in cold (4℃) UW (group 1, n=8) or EC (group 2, n=9) solutions for 16 hours. The preserved (group 1 and 2) and control (group 3, n=7) pulmonary arterial segments with and without endothelium were studied in vitro in organ chambers to measure isometric tension.Results The endothelium-dependent relaxation to acetylcholine and adenosine diphosphate of group 1 and 3 were significantly better than those of group 2.Conclusions We concluded that endothelium-dependent relaxation of canine pulmonary arterial endothelium to receptor-dependent acetylcholine and adenosine diphosphate were impaired after preservation with Euro-Collins solution. However, endothelium-dependent relaxation of pulmonary segments were well maintained after preservation with University of Wisconsin solution.
