Untargeted metabolomic analysis of serum samples from children with mycoplasma pneumonia in a hospital in Beijing
10.3760/cma.j.cn112150-20210310-00237
- VernacularTitle:北京某医院儿童肺炎支原体肺炎患者血清非靶向代谢组学分析
- Author:
Qin HUI
1
;
Qi ZHANG
;
Kundi WANG
;
Jing ZHANG
;
Chengning ZHENG
;
Zhongshu ZHOU
Author Information
1. 中国医学科学院北京协和医学院研究生院 100070
- Keywords:
Mycoplasma pneumoniae;
Metabolome;
Metabolites pathway
- From:
Chinese Journal of Preventive Medicine
2021;55(5):660-666
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study was aimed to analyze the untargeted metabolomics of serum samples from children with mycoplasma pneumonia in a hospital in Beijing.Methods:A total of 50 children with mycoplasma pneumonia as the case group were recruited from Department of Pediatrics, China-Japan Friendship Hospital in Beijing from January 2019 to February 2020, and meanwhile 50 age-and gender-matched heathy children were selected and formed the control group. 2 ml venous fasting blood samples was collected from all children. Serum metabolites were quantified by using the untargeted ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) technique. Unsupervised principle component analysis and (orthogonal) partial least-squares-discriminant analysis were employed to identify differential metabolites between cases and controls. MBRole software was used for pathway enrichment analysis.Results:There were 27 boys and 23 girls in the case group with an average age of (6.0±3.65) years, and the control group consisted of 28 boys and 22 girls with an average age of (6.62±2.64) years. A total of 392 different metabolites were detected. Compared with the control group, 306 metabolites were decreased and 86 increased in case group. Forty-one differential metabolites with variable important in projection (VIP) values larger than 5 and P values less than 0.05 were teased out, and they mainly concentrated on phospholipid. The levels of 38 metabolites were significantly lower in the case group, yet 4 metabolites were significantly higher than that of the control group. Metabolic enrichment analysis showed that different metabolites were related to the biosynthesis of phenylalanine, tyrosine, tryptophan, unsaturated fatty acid, ammonia acyl tRNA and insulin signaling pathway, as well as the metabolism of ABC transporters. Conclusion:The serum untargeted metabolomics differed remarkably between children with mycoplasma pneumonia and healthy children.
