Isolation and Identification of two Escherichia albertii strains in Shanxi Province, China
10.3760/cma.j.cn112150-20200429-00667
- VernacularTitle:山西省内2株艾伯特埃希菌的分离与鉴定
- Author:
Jitao WANG
1
;
Dequan MENG
;
Jiane GUO
;
Guodong YAN
;
Ling ZHANG
;
Ziting WAN
;
Qun LI
;
Li GAO
;
Ruihong GAO
;
Lifeng ZHAO
;
Hong WANG
Author Information
1. 太原市疾病预防控制中心微生物检验科 030001
- Keywords:
Sequence typing;
Escherichia albertii;
Polymerase chain reaction
- From:
Chinese Journal of Preventive Medicine
2020;54(10):1121-1126
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the prevalence of Escherchia albertii in Shanxi province. Methods:The chicken intestines were enriched in EC broth. The eae gene was detected by PCR, and the eae-positive EC enrichments were inoculated in MacConkey agar plate. The eae-positive lactose non-fermenting isolates were presumed as Escherchia albertii, and then analyzed by triplex-PCR, 16S rDNA sequencing and MLST. Results:Two suspected Escherchia albertiiwere isolated from 250 samples of chicken intestines. It was identified as Escherchia albertii by phenotypic, specific genes,16S rDNA sequencing, and MLST analyses . The cytolethal distending toxin B ( cdtB) showed positive by PCR,and they were clusted to Ⅱ/Ⅲ/Ⅴ group by sequencing. Conclusion:This study showed that the Escherchia albertii was existed in Shanxi province, China.
