Analysis of infection status and recombination types of norovirus in patients with acute gastroenteritis in the Ningxia Hui Autonomous Region from 2016 to 2017
10.3760/cma.j.issn.0253?9624.2019.08.003
- VernacularTitle:2016—2017年宁夏急性胃肠炎患者中诺如病毒感染状况及重组类型分析
- Author:
Wen DANG
1
;
Jiangtao MA
;
Hui CHEN
;
Fang YUAN
;
Xuemin MA
;
Jun ZHAN
Author Information
1. 宁夏医科大学公共卫生与管理学院
- Keywords:
Norovirus;
Genotype;
Surveillance;
Recombination
- From:
Chinese Journal of Preventive Medicine
2019;53(8):811-816
- CountryChina
- Language:Chinese
-
Abstract:
To analyze the infection status and recombination of Norovirus in patients with acute gastroenteritis in Ningxia. Methods The specimens of 10 sentinel hospitals in Ningxia were collected from 2016 to 2017. Real?time quantitative PCR was used for nucleic acid detection. GⅡ?positive samples were amplified by RT?PCR for the RdRp and Capsid regions, then sequenced and genotyped. Evolution analysis was performed using software such as MEGA?X, and recombination analysis was performed using Simplot 3.5.1 and RDP4.Results The age of the 2 334 cases was 1.42 (0.68, 7.69) years old, 1 133 cases in 2016 and 1 201 cases in 2017, 1 343 and 991 cases for males and females respectively. The positive rate of Norovirus GⅠ genogroup was 0.86% (20/2 334), and GⅡ genogroup was 14.82% (346/2 334). A total of 78 recombinant strains were sequenced and 12 recombinant types were found. GⅡ. Pe/GⅡ. 4Sydney_2012 and GⅡ. P12/GⅡ. 3 were the main epidemic strains, accounting for 35.90% (28 strains) and 32.05% (25 strain) respectively, followed by GⅡ. P16/GⅡ. 2 accounting for 12.82% (10 strains). Among them,GⅡ.P7/GⅡ.6 (2 strains), GⅡ.P12/GⅡ.3 (6 strains), GⅡ.P16/GⅡ.1 (2 strains), GⅡ. P16/GⅡ.2 (5 strains), GⅡ.Pe/GⅡ.4 (7 strains) were detected for the first time in Ningxia. Recombinant strains were all intergenotype recombination, and the recombination breakpionts were all located within ORF1. Conclusion Norovirus infection in Ningxia area was mainly in GⅡgenogroup from 2016 to 2017, and most of them were recombinant strains. GⅡ.Pe/GⅡ.4Sydney_2012 and GⅡ.P12/GⅡ.3 were the main epidemic strains, followed by GⅡ.P16/GⅡ. 2.
