Genetic characterization of varicella zoster virus in Jilin province in 2014, China
10.3760/cma.j.issn.0253-9624.2016.08.013
- VernacularTitle:2014年吉林省水痘-带状疱疹病毒基因特征分析
- Author:
Qiuhua WU
1
;
Shuang WANG
;
Xianling CONG
;
Jianhui ZHOU
;
Aili CUI
;
Wenbo XU
;
Songtao XU
Author Information
1. 中国疾病预防控制中心病毒病预防控制所卫生部医学病毒和病毒病重点实验室
- Keywords:
Varicellovirus;
Genotype;
Vaccines;
Wild-type strain
- From:
Chinese Journal of Preventive Medicine
2016;50(8):738-742
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clarify the genotype of wild-type strains of varicella zoster virus (VZV) in Jilin province in 2014,and to discriminate between v-Oka vaccine strains and wild-type strains.Methods Vesicle fluid and throat swab samples were collected from 13 individuals with suspected VZV in Jilin province from January to December 2014.Viral DNA was extracted,the fragments of 15 open reading fragments (ORFs) were amplified by polymerase chain reaction (PCR),and viral genotypes were determined by single nucleotide polymorphisms (SNP).PCR restriction fragment length polymorphism (RFLP) was used to distinguish between wild-type strains and v-Oka vaccine strains.The results were analyzed with MEGA5 software,using the VZV reference strain sequences from GenBank.Results The 13 suspected samples included 5 males and 8 females,aged 11-27 years (mean:(16.69±5.48) years).Sampling was performed on days 0 to 3 of suspected infection.VZV strains were detected in 8 samples,all belonging to Clade 2.There was a synonymous mutation (T＞C) in SNP18082 compared with the v-Oka vaccine strain.Analysis of PCR-RFLPs showed that all 8 positive samples were wild-type strains (Pst Ⅰ +Bgl Ⅰ +Sma Ⅰ-).Conclusions The study revealed that the VZV strains circulating in Jilin province in 2014 were wild-type strains belonging to Clade 2.
