In vivo Tracking of Mesenchymal Stem Cells Labeled with a Novel Chitosan-coated Superparamagnetic Iron Oxide Nanoparticles using 3.0T MRI.
10.3346/jkms.2010.25.2.211
- Author:
Alavala Matta REDDY
1
;
Byung Kook KWAK
;
Hyung Jin SHIM
;
Chiyoung AHN
;
Hyo Sook LEE
;
Yong Jae SUH
;
Eon Sub PARK
Author Information
1. Department of Radiology, Chung-Ang University College of Medicine, Seoul, Korea. kwakbk@cau.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Chitosan;
Mesenchymal Stem Cells;
Superparamagnetic Iron Oxide;
Magnetic Resonance Imaging;
Brain Ischemia
- MeSH:
Animals;
Brain Ischemia/chemically induced/pathology/therapy;
Cell Differentiation;
Chitosan/*chemistry;
Coordination Complexes/*chemistry/toxicity;
Ferric Compounds/*chemistry;
Humans;
Magnetic Resonance Imaging;
Magnetics;
Mesenchymal Stem Cell Transplantation;
Mesenchymal Stromal Cells/*chemistry/cytology;
Metal Nanoparticles/*chemistry;
Phenotype;
Rabbits
- From:Journal of Korean Medical Science
2010;25(2):211-219
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study aimed to characterize and MRI track the mesenchymal stem cells labeled with chitosan-coated superparamagnetic iron oxide (Chitosan-SPIO). Chitosan-SPIO was synthesized from a mixture of FeCl2 and FeCl3. The human bone marrow derived mesenchymal stem cells (hBM-MSC) were labeled with 50 microg Fe/mL chitosan-SPIO and Resovist. The labeling efficiency was assessed by iron content, Prussian blue staining, electron microscopy and in vitro MR imaging. The labeled cells were also analyzed for cytotoxicity, phenotype and differentiation potential. Electron microscopic observations and Prussian blue staining revealed 100% of cells were labeled with iron particles. MR imaging was able to detect the labeled MSC successfully. Chitosan-SPIO did not show any cytotoxicity up to 200 microgram Fe/mL concentration. The labeled stem cells did not exhibit any significant alterations in the surface markers expression or adipo/osteo/chondrogenic differentiation potential when compared to unlabeled control cells. After contralateral injection into rabbit ischemic brain, the iron labeled stem cells were tracked by periodical in vivo MR images. The migration of cells was also confirmed by histological studies. The novel chitosan-SPIO enables to label and track MSC for in vivo MRI without cellular alteration.
