Epidemic condition and molecular subtyping of ciprofloxacin and cefotaxime co-resistant Salmonella Indiana isolated from retail chicken carcasses in six provinces, China
10.3760/cma.j.issn.0253-9624.2015.08.010
- VernacularTitle:中国六省份零售整鸡中环丙沙星与头孢噻肟双耐药印第安纳沙门菌流行状况及分子分型研究
- Author:
Yujie HU
1
;
Yingying HE
;
Yeru WANG
;
Shenghui CUI
;
Qiuxia CHEN
;
Guihua LIU
;
Qian CHEN
;
Gang ZHOU
;
Baowei YANG
;
Jinlin HUANG
;
Hongxia YU
;
Fengqin LI
Author Information
1. 国家食品安全风险评估中心卫生部食品安全风险评估重点实验室
- Keywords:
Salmonella;
Ciprofloxacin;
Cefotaxime;
Electrophoresis,gel,pulsed-field;
Drug resistance
- From:
Chinese Journal of Preventive Medicine
2015;(8):716-721
- CountryChina
- Language:Chinese
-
Abstract:
Objective To elucidate the epidemic condition and molecular subtyping of ciprofloxacin and cefotaxime co-resistant Salmonella Indiana(S. Indiana)isolated from retail chicken carcasses in six provinces of China. Methods A total of 2 647 Salmonella strains isolated from retail chicken carcasses collected from six provinces of China were subjected to antimicrobial susceptibility testing. All Salmonella isolates co-resistant to ciprofloxacin and cefotaxime were further characterized by serotyping, extended-spectrum beta-lactamases (ESBLs) producing strains screening and pulsed field gel electrophoresis (PFGE) typing. Results Among 2 629 Salmonella isolates tested, 227 (8.52%) isolates were co-resistant to ciprofloxacin and ceftazidime/cefotaxime (Beijing:11.67%(99/874),Jilin:8.20%(60/726), Guangdong: 1.39%(7/502),Jiangsu: 15.61%(42/260),Shaanxi: 8.56%(16/186),Inner Mongolia: 0(0/81)), and 224 of them were identified as S. Indiana. 213(95.10%)isolates of S. Indiana were ESBLs producing strains. All ciprofloxacin and cefotaxime co-resistant S. Indiana isolates developed a multi-drug resistant profile and 17.86%(40/224)of them were resistant to all antibiotics tested except carbapenems, and 50.89%(114/224)of them resistant to 9 antibiotics, additionally, 25.45%(57/224)of them showed multi-drug resistance to 8 antibiotics. All ciprofloxacin and cefotaxime co-resistant S. Indiana isolates were divided into 32 PFGE clusters and 150 PFGE patterns. Strains of S. Indiana from same or different sampling site and time seemed to either share the same PFGE patterns or be differential to each other in different regions. Conclusion The results indicated that chicken carcasses collected from parts of China were heavily contaminated by ciprofloxacin and cefotaxime co-resistant S. Indiana and could serve as an important reservoir of ciprofloxacin and cefotaxime co-resistant Salmonella. Molecular subtyping results indicated that cross contamination or common pollution source might be in these strains.
