Genotoxic effects of oral-exposed TiO2 nanoparticles on bone marrow cells in young rats

VernacularTitle:纳米二氧化钛短期经口染毒对幼年大鼠骨髓细胞的遗传损伤作用
Author: Yun WANG ¹ ; Zhangjian CHEN ; Te BA ; Ji PU ; Yongen GU ; Jian GUO ; Guang JIA
Author Information

1. 100191,北京大学医学部公共卫生学院劳动卫生与环境卫生学系
Keywords: Nanoparticles; Titanium; Rats; DNA damage; Safety
From: Chinese Journal of Preventive Medicine 2014;(9):815-818
CountryChina
Language:Chinese
Abstract: Objective To explore the genotoxic effects of oral-exposed TiO2 nanoparticles on bone marrow cells in young rats.Methods Twenty-eight SD male young rats ( 4 weeks old ) were randomly divided into 4 groups, which were exposed to TiO2 nanoparticles ((75 ±15) nm, anatase) through intragastric administration at 0, 10, 50 and 200 mg/kg body weight ( bw) every day for 30 days.The bone marrow cells were collected for micronuclei and γ-H2 AX immunofluorescence analysis.Results The percentage of γ-H2 AX foci-positive cells (37.4 ±10.0)%in the 50 mg/kg bw dose group were significantly higher than that in the control group (19.8 ±3.1)% (t value was -17.59, P<0.01).No significantly difference was found in polychromatic erythrocyte/normochromatic erythrocyte ( PCE/NCE) ratio and PCE micronucleus rate between three experimental groups and control group.Conclusion TiO2 nanoparticles can increase the frequency of DNA double-strand breaks in bone marrow cells , but has no effect on micronucleus of bone marrow cells in young rats.