The relationship between cadmium-induced inhibition of splenic lymphocyte function and cell apoptosis
10.3760/j:issn:0253-9624.2001.01.016
- VernacularTitle:镉致脾淋巴细胞功能抑制与细胞凋亡的关系
- Author:
Feng FENG
1
;
Xian ZHANG
;
Bin XUE
Author Information
1. 北京大学
- From:
Chinese Journal of Preventive Medicine
2001;35(1):44-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between cadmium-induced inhibition of splenic lymphocyte function and cell apoptosis in vitro.Methods The splenic lymphocytes of mice were exposed to 3.10, 6.25, 12.50, 25.00, 50.00 μmol/L of cadmiun chloride (CdCl2) for various time period, to measure the lymphocyte transformation by MTT colorimetric assay and to detect the apoptosis in splenocytes by DNA agarose electrophoresis and flow cytometry (FACS).Results CdCl2 could significantly inhibit the function of lymphocyte transformation in a dose-response pattern at concentrations of 25.00 and 50.00 μmol/L, with inhibition rates of 50% and 78% in ConA-stimulating group and 39% and 55% in LPS-stimulating group, respectively.CdCl2 could also induce apoptosis of splenic cells at concentrations of 12.50 to 50.00 μmol/L.Results of FACS analysis showed 30%-60% of cell apoptosis.Higher concentration of CdCl2 could also cause reduction of cell survival.Effect of Cd-induced apoptosis was earlier and at a lower concentration of CdCl2 than that of Cd-induced inhibition of lymphocyte transformation.Conclusion CdCl2 could induce cell apoptosis in vitro, which was one of the mechanisms of its suppression of lymphocyte function.
