An animal model of bladder reconstruction by autologous peritoneum transplantation
10.3760/cma.j.issn.0529?5815.2019.11.011
- VernacularTitle:腹膜自体移植回肠浆肌层重建新膀胱的动物模型建立
- Author:
Jun SHEN
1
;
Junfeng WU
;
Ju ZHANG
;
Zhiqiang SONG
;
Shengwen LI
Author Information
1. 贵州医科大学附属医院泌尿外科
- Keywords:
Urinary bladder;
Reconstructive surgical procedures;
Peritoneum;
Ileum;
Swine
- From:
Chinese Journal of Surgery
2019;57(11):853-859
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish experimental porcine model of reconstruction the neobladder by ileal seromuscular with transplantation of autologous peritoneum. Methods This was an animal experiment carried out from January to April 2018 at animal center of Guizhou Medical University. Randomly 6 experimental female porcines were chosen, and their body weight was 28 to 33 kg. By intravenous anesthesia, the transplantation of autologous peritoneum for bladder reconstruction operation was carried out by transplanting the peritoneum onto an ileum segment which mucosa and submucosa had been removed. These flaps were used to mend and reconstruct the neobladder by suturing with edge of the detective bladder. After removal of ureteral catheters and balloon catheter at day 5 and day 7 respectively, voiding behavior was monitored, and animals were euthanized at week 12 for routine pathology, immunohistochemistry, and electron microscopic examinations. Results Six porcines underwent reconstruction, but no one lost to complications such as peritonitis, ileus and urinary fistula. Voiding behavior was normal, and urine was clear in all animals after removal of catheters. At autopsy, reconstructed bladders were healthy. Pathological examination showed the part of reconstruction had been covered by continuous urothelium while the peritoneum disappeared and showed no ileal mucosa regrowth and residual. Scanning electron microscope showed that the transitional cells of neobladder were complete and orderly, and urothelium around suture border was continuous and no malposition. Conclusion In this experimental porcine model, reconstruction bladder by autologous peritoneum and ileal seromuscular flaps is an ideal approach.
