Effect of donor age on the proliferation and multipotency of canine adipose-derived mesenchymal stem cells.
10.4142/jvs.2017.18.2.141
- Author:
Jienny LEE
1
;
Keum Sil LEE
;
Chan Lan KIM
;
Jeong Su BYEON
;
Na Yeon GU
;
In Soo CHO
;
Sang Ho CHA
Author Information
1. Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea. virusmania@korea.kr
- Publication Type:Original Article
- Keywords:
adipose mesenchymal stem cells;
age;
canine;
differentiation;
multipotency
- MeSH:
Adipose Tissue;
Animals;
Cell Survival;
Dogs;
Humans;
Kinetics;
Mesenchymal Stromal Cells*;
Tissue Donors*
- From:Journal of Veterinary Science
2017;18(2):141-148
- CountryRepublic of Korea
- Language:English
-
Abstract:
Research into adipose tissue-derived mesenchymal stem cells (AD-MSCs) has demonstrated the feasibility of their use in clinical applications due to their ease of isolation and abundance in adipose tissue. We isolated AD-MSCs from young and old dogs, and the cells were subjected to sequential sub-passaging from passage 1 (P1) to P7. Canine AD-MSCs (cAD-MSCs) were examined for proliferation kinetics, expression of molecules associated with self-renewal, expression of cell surface markers, and differentiation potentials at P3. Cumulative population doubling level was significantly higher in cAD-MSCs of young donors than in those of old donors. In addition, expressions of CD73, CD80, Oct3/4, Nanog, cell survival genes and differentiation potentials were significantly higher in young donors than in old donors. The present study suggests that donor age should be considered when developing cell-based therapies for clinical application of cAD-MSCs.
