Estrogen receptor-α, progesterone receptor, and c-erbB/HER-family receptor mRNA detection and phenotype analysis in spontaneous canine models of breast cancer.
10.4142/jvs.2017.18.2.149
- Author:
Farruk M. Lutful KABIR
1
;
Patricia DEINNOCENTES
;
Payal AGARWAL
;
Christopher P MILL
;
David J RIESE
;
R Curtis BIRD
Author Information
1. Auburn University Research Initiative in Cancer (AURIC), Department of Pathobiology, College of Veterinary Medicine, Harrison School of Pharmacy, Auburn University, AL 36849, USA. birdric@auburn.edu
- Publication Type:Original Article
- Keywords:
breast cancer;
canine;
mammary epithelial cells;
estrogen/progesterone receptor;
c-erbB/HER-2 receptor
- MeSH:
Breast Neoplasms*;
Breast*;
Cell Line;
Classification;
Epithelial Cells;
Estrogen Receptor alpha;
Estrogens*;
Humans;
Phenobarbital;
Phenotype*;
Progesterone*;
Proto-Oncogenes;
Receptors, Progesterone*;
Reverse Transcriptase Polymerase Chain Reaction;
RNA, Messenger*;
Sequence Analysis, DNA
- From:Journal of Veterinary Science
2017;18(2):149-158
- CountryRepublic of Korea
- Language:English
-
Abstract:
Well characterized, stable, p16-defective canine mammary cancer (CMT) cell lines and normal canine mammary epithelial cells were used to investigate expression of the major breast cancer-specific hormone receptors estrogen receptor alpha (ER1) and progesterone receptor (PR) as well as luminal epithelial-specific proto-oncogenes encoding c-erbB-1 (epidermal growth factor receptor/EGFr), c-erbB-2/HER2, c-erbB-3, and c-erbB-4 receptors. The investigation developed and validated quantitative reverse transcriptase polymerase chain reaction assays for each transcript to provide rapid assessment of breast cancer phenotypes for canine cancers, based on ER1, PR, and c-erbB-2/HER2 expressions, similar to those in human disease. Roles for relatively underexplored c-erbB-3 and c-erbB-4 receptor expressions in each of these breast cancer phenotypes were also evaluated. Each quantitative assay was validated by assessment of amplicon size and DNA sequencing following amplification. Differential expression of ER1, PR, and c-erbB-2 in CMT cell lines clearly defined distinct human-like breast cancer phenotypes for a selection of CMT-derived cell lines. Expression profiles for EGFr family genes c-erbB-3 and c-erbB-4 in CMT models also provided an enriched classification of canine breast cancer identifying new extended phenotypes beyond the conventional luminal-basal characterization used in human breast cancer.
