Immunogenicity of recombinant Lactobacillus plantarum NC8 expressing goose parvovirus VP2 gene in BALB/c mice.
10.4142/jvs.2017.18.2.159
- Author:
Yu Ying LIU
1
;
Wen Tao YANG
;
Shao Hua SHI
;
Ya Jie LI
;
Liang ZHAO
;
Chun Wei SHI
;
Fang Yu ZHOU
;
Yan Long JIANG
;
Jing Tao HU
;
Wei GU
;
Gui Lian YANG
;
Chun Feng WANG
Author Information
1. College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun 130118, China. yangguilian@jlau.edu.cn, wangchunfeng@jlau.edu.cn
- Publication Type:Original Article
- Keywords:
Lactobacillus plantarum;
VP2 gene;
goose parvovirus;
immunization
- MeSH:
Agriculture;
Animals;
Geese;
Immunization;
Immunoglobulin A, Secretory;
Interferons;
Lactobacillus plantarum*;
Lactobacillus*;
Lymphocytes;
Mice*;
Molecular Weight;
Parvovirus*;
Spleen;
Tumor Necrosis Factor-alpha;
Vaccines
- From:Journal of Veterinary Science
2017;18(2):159-167
- CountryRepublic of Korea
- Language:English
-
Abstract:
Goose parvovirus (GPV) continues to be a threat to goose farms and has significant economic effects on the production of geese. Current commercially available vaccines only rarely prevent GPV infection. In our study, Lactobacillus (L.) plantarum NC8 was selected as a vector to express the VP2 gene of GPV, and recombinant L. plantarum pSIP409-VP2/NC8 was successfully constructed. The molecular weight of the expressed recombinant protein was approximately 70 kDa. Mice were immunized with a 2 × 109 colony-forming unit/200 µL dose of the recombinant L. plantarum strain, and the ratios and numbers of CD11c⁺, CD3⁺CD4⁺, CD3⁺CD8⁺, and interferon gamma- and tumor necrosis factor alpha-expressing spleen lymphocytes in the pSIP409-VP2/NC8 group were higher than those in the control groups. In addition, we assessed the capacity of L. plantarum SIP409-VP2/NC8 to induce secretory IgA production. We conclude that administered pSIP409-VP2/NC8 leads to relatively extensive cellular responses. This study provides information on GPV infection and offers a clear framework of options available for GPV control strategies.