Alterations in antioxidant function and cell apoptosis in duck spleen exposed to molybdenum and/or cadmium.
10.4142/jvs.2017.18.2.193
- Author:
Mengmeng ZHANG
1
;
Junrong LUO
;
Caiying ZHANG
;
Huabin CAO
;
Bing XIA
;
Guoliang HU
Author Information
1. Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agriculture University, Nanchang 330045, China. zhangcaiying0916@163.com
- Publication Type:Original Article
- Keywords:
apoptosis;
cadmium;
duck;
molybdenum;
oxidative stress
- MeSH:
Apoptosis*;
Cadmium*;
Caspase 3;
Catalase;
Diet;
Ducks*;
Malondialdehyde;
Molybdenum*;
Oxidative Stress;
RNA, Messenger;
Spleen*;
Xanthine Oxidase
- From:Journal of Veterinary Science
2017;18(2):193-200
- CountryRepublic of Korea
- Language:English
-
Abstract:
To investigate the effects of molybdenum (Mo) and/or cadmium (Cd) on antioxidant function and the apoptosis-related genes in duck spleens. Sixty healthy 11-day-old ducks were randomly divided into six groups of 10 ducks (control, low Mo group, high Mo, Cd, low Mo + Cd, and high Mo + Cd groups). All were fed a basal diet containing low or high dietary doses of Mo and/or Cd. Relative spleen weight, antioxidant indices, apoptosis-related gene mRNA expression levels, and ultrastructural changes were evaluated after 120 days. The results showed that the relative spleen weight decreased significantly in the high Mo + Cd treatment group which compared with control group. Malondialdehyde levels increased and xanthine oxidase and catalase activities decreased in the Mo and/or Cd groups compared with levels in the control group. Bak-1 and Caspase-3 expressions were upregulated in the high Mo + Cd group, while Bcl-2 was downregulated. In addition, mitochondrial crest fracture, swelling, vacuolation, deformed nuclei, and karyopyknosis in both Mo + Cd treated groups were more severe than in the other groups. The results suggest that Mo and/or Cd can induce oxidative stress and apoptosis of spleen via effects on the mitochondrial intrinsic pathway. Moreover, the results indicate the two elements have a possible synergistic relationship.
